In BC, urinary cell-free NAs could be excreted immediately by cancer tissues in the form of exosomes. Thus, elevated expression of certain genes in cancer tissues might be mirrored by elevated expression in the urine. Another advantage is that we in contrast PicoGreen- and -RiboGreen-adjusted expression of urinary cell-free NAs. No earlier examine has straight in contrast PicoGreen- and RiboGreen-adjusted expression of urinary cell-free NAs. There are a number of limitations in this examine. Unfortunately, there was no experiment to explain the tissue specificity of urinary NAs of IQGAP3. Further studies are essential to verify the tissue specificity of NAs of IQGAP3 in BC. And though this research compared immediately PicoGreen-and RiboGreen-adjusted expression of urinary cell-free NAs, there was no study which adjustment of urinary NAs is suitable. Additional research are necessary to confirm which adjustment of urinary NAs is acceptable. Second, because there were no results of cytology, we did not compare the results of cytology and urinary NAs. Third, there was no validation study to affirm as a diagnostic marker in one other cohort. Additional validation research with one other cohort patients with cytology are crucial. In conclusion, IQGAP3 urinary cell-free NAs in BC patients had been considerably greater than those in regular controls or in patients with hematuria. High expression of IQGAP3 urinary cell-free NAs in BC reflects excessive expression in BC tissue. Therefore, IQGAP3 urinary cell-free NAs could also be a complementary diagnostic biomarker for BC.
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